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. 2006 Dec 5;4(12):e435. doi: 10.1371/journal.pbio.0040435

Figure 7. HIV-1 Assembly Does Not Required Microtubules or Endosome Motility in Primary Human Macrophages.

Figure 7

(A) Macrophages expressing Gag-GFP were treated with nocodazole or U18666A for the entire duration of Gag-GFP synthesis. Cells were fixed at 12-h post-transfection and stained with Hoechst 33258 (blue). Scale bars indicate 10 μm.

(B) Western blot analysis of macrophages expressing Gag-GFP in the presence of DMSO, nocodazole, or U18666A. Samples were collected every 2 h, from 4- to 12-h post-transfection. Cell and virion lysates were probed with anti–HIV-1 CA antibodies. Numerical values below the blots indicate VLP signal intensities, derived by densitometry.