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. 2006 Sep 13;10(5):R130. doi: 10.1186/cc5042

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Copyright © 2006 Mura et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Intestinal ischemia reperfusion (IIR)-induced alveolar epithelial cell death is negatively correlated with vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor (VEGFR)-1 expression. (a) Double fluorescents staining TUNEL (red)-cytokeratin (green). An increased number of epithelial cells (in green) undergoing cell death (in red) was detected in the IIR group. Slides shown are representatives of four animals from each group. (b) Epithelial cell death index was quantified by TUNEL-cytokeratin double positive cells over cytokeratin positive cells of each field (ten fields were quantified from each animal); *p < 0.05 compared with the control group; ^#p < 0.05 compared with the sham group. (c) Relationship between TUNEL-positive-epithelial cells and VEGF-positive cells. (d) Relationship between TUNEL-positive-epithelial cells and VEGFR-1-positive cells.