Table 1.

Rat neutrophil chemotaxis and adhesion in vitro induced by SEA

Chemotaxis ( × 105/ml)		Adhesion (%)
MEM/Control	7.13±0.62	MEM/Control	49.5±0.86
fMLP (10−7 M)	11.1±0.40*	fMLP (10−5 M)	71.6±0.89*
SEA (0.01 ng well−1)	4.90±0.64	SEA (0.1 ng well−1)	55.5±0.14
SEA (0.10 ng well−1)	6.50±0.40	SEA (0.3 ng well−1)	48.6±1.91
SEA (1.00 ng well−1)	8.00±0.51	SEA (1.0 ng well−1)	53.8±0.87

Neutrophils were isolated from rat peripheral blood in 3.13% (w v⁻¹) sodium citrate (10 : 1) and obtained by dextran sedimentation using Ficol (1.077 g l⁻¹) gradient (see Methods).

The myeloperoxidase (MPO) activity was measured, and results were expressed as absolute number of neutrophil that migrated through the filter (chemotaxis) or % neutrophil adhesion. The data represent the mean values±s.e.m. of three experiments (each in duplicate).

^*P<0.05 compared with respective MEM.