Figure 7.

Proinsulin biosynthesis in islets from prediabetic, normoglycaemic P. obesus. Islets were incubated for 2 h (a) or 24 h (b) with 1.7 or 8.3 mM glucose (G 1.7 and G 8.3, respectively) in the absence (Control, open columns) or presence (Test, filled columns) of 1 μM S 21403. To evaluate the rate of proinsulin biosynthesis, leucine incorporation into islet insulin-like material is shown (see Methods for details). Mean±s.e.m. of 4 experiments, each using islets pooled from 2 animals.