Table 3.

Chronic effects of S 21403 and glucose in islets from prediabetic P. obesus

Culture condition	G 5.5	G 5.5+S	G 33	G 33+S
Islet insulin contenta (pmol islet−1)	1.05±0.21	0.87±0.12	0.40±0.02*	0.48±0.02**
Secretion at G 3.3b (pmol islet−1 h−1)	0.092±0.024	0.057±0.001	0.124±0.009	0.0116±0.009
Secretion at G 16.7b (pmol islet−1 h−1)	0.133±0.026†	0.118±0.028‡	0.032±0.003	0.052±0.004§

Results are mean±s.e.m. of four culture plates in glucose alone (G, 5.5 or 33 mM) or with the addition of 1 μM S 21403 (S) for 1 week.

^aIslet insulin content was determined after the culture period and following the 1-h acute incubation at 3.3 mM glucose; the values shown are islet content+the insulin secreted during the 1-h incubation.

^bThe 1-h incubation in 3.3 or 16.7 mM glucose at the end of the culture period (see Methods).

^*P=0.0019 vs G 5.5.

^**P=0.0506 vs G 33.

^†P=0.3 vs G 3.3.

^‡P=0.07 vs G 3.3 in G 5.5+S.

^§P=0.0096 vs G 16.7 in G 33.