Table 1.
Physiological parameters
| Parameter | UNX (n=10) | UNX+A-127722 (n=8) | DOCA-salt 14 days (n=6) | DOCA-salt 28 days (n=10) | DOCA-salt+A-127722 28 days (n=8) |
|---|---|---|---|---|---|
| Whole animal | |||||
| Initial body weight (g) | 302±4 | 307±2 | 305±4 | 307±5 | 310±4 |
| Final body weight (g) | 376±11 | 399±13 | 325±6 | 312±8 | 344±7 |
| Heart rate (b.p.m.) | 390±12 | 382±19 | 401±23 | 397±17 | 368±20 |
| Systolic blood pressure (mmHg) | 128±6 | 127±3 | 158±3* | 182±5*# | 150±6** |
| Organ weights | |||||
| Left ventricular weight (mg g−1 body wt) | 1.99±0.06 | 2.20±0.05 | 2.48±0.05* | 3.30±0.08*# | 2.65±0.06**# |
| Right ventricular weight (mg g−1 body wt) | 0.56±0.02 | 0.54±0.04 | 0.56±0.02 | 0.65±0.03* | 0.63±0.03* |
| Kidney weight (mg g−1 body wt) | 4.98±0.11 | 4.65±0.08 | 7.76±0.33* | 10.90±0.40* | 10.03±0.55*# |
| Histology | |||||
| Thoracic aortic ring wall thickness (μm) | 192±7 | 196±8 | 236±4* | 312±5*# | 228±3** |
| Left ventricular perivascular collagen fraction (% area) | 27.9±4.4 | 32.3±3.9 | 37.8±2.1* | 38.9±2.8* | 32.7±1.9** |
| Left ventricular interstitial collagen fraction (% area) | 2.7±0.3 | 4.2±1.9 | 6.7±2.4* | 11.7±1.3*# | 7.6±1.3** |
| Kidney interstitial collagen fraction (% area) | 22.4±3.6 | 19.8±3.7 | 30.9±2.2* | 39.1±3.6* | 32.4±4.8* |
| Function | |||||
| Diastolic stiffness | 21.1±0.5 | 22.5±1.0 | 24.6±1.8* | 30.1±1.3*# | 22.1±1.2** |
| Maximum +dP/dt (mmHg s−1) | 1790±140 | 1630±100 | 1670±80 | 1380±170*# | 1440± 100* |
| Minimum −dP/dt (mmHg s−1) | 1490±70 | 1500±130 | 1500±110 | 1310±150 | 1250±110 |
| Echocardiography | |||||
| LVIDd (mm) | 6.69±0.18 | 7.17±0.16 | 6.29±0.16 | 5.51±0.37*# | 6.39±0.31** |
| LVPWd (mm) | 1.74±0.17 | 1.78±0.17 | 1.79±0.07 | 1.98±0.09 | 1.85±0.09 |
| E/A (early/atrial) mitral valve flow ratio | 1.92±0.02 | 1.88±0.07 | 1.82±0.27 | 1.64±0.10* | 1.88±0.10** |
| Ejection fraction (%) | 83.1±4.4 | 82.7±5.6 | 94.6±1.1* | 92.7±1.2* | 94.8±1.3* |
| Cardiac output (ml min−1) | 75.3±3.6 | 72.1±6.8 | 63.9±7.8 | 53.7±6.7* | 58.1±5.3* |
| Ascending aorta (m s−1) | 0.75±0.08 | 0.88±0.10 | 1.12±0.07* | 1.23±0.05* | 0.98±0.06** |
| Left ventricular mass (g) (Litwin et al., 1994) | 0.76±0.04 | 0.85±0.09 | 0.75±0.03 | 1.36±0.09*# | 0.99±0.08**# |
All values shown represent the mean±s.e.m.; LVIDd=left ventricular internal diameter in diastole; LVPWd=left ventricular posterior wall thickness in diastole.
*
P<0.05 vs UNX rats;
**
P<0.05 vs DOCA-salt rats.