Figure 7.

The distribution of PKCα (a), PKCδ (b) and PKCɛ (c) in mesenteric arteries from control (C) and diabetic rats (D) in the absence (UT) and presence of ET-1 (0.03 μM for 7 min.). A representative Western blot showing cytosolic (C) and particulate (P) fractions is shown, together with a graph showing the mean±s.e.m. of data obtained from five different preparations. The first band on each blot represents the purified standard of the isoform (Std). Cytosolic (open bars) and particulate (closed bars) levels of PKC isoforms following ET-1 treatment were calculated relative to levels of the same isoform in the corresponding fraction from untreated control arteries. ^*P<0.05 compared to the corresponding untreated group; ⁺P<0.05 compared to the corresponding control untreated group; ^@P<0.05 compared to the corresponding control ET-1-treated group (two-way ANOVA followed by Newman–Keuls post hoc test).