Table 2.
NA, ET-1 and PdBu Rmax and pD2 values in mesenteric arteries from control and diabetic rats
| Control | Diabetic | |||
|---|---|---|---|---|
| Rmax | pD2 | Rmax | pD2 | |
| g | –log EC50 | g | −log EC50 | |
| Noradrenaline | ||||
| Untreated (13) | 1.35±0.10 | 6.84±0.1 | 1.86±0.09* | 7.13±0.13* |
| Ro-318220 | 1.15±0.09 | 6.40±0.06** | 1.44±0.09** | 6.63±0.04** |
| Untreated (8) | 1.51±0.09 | 6.81±0.06 | 1.93±0.08* | 7.11±0.08* |
| Calphostin C | 1.43±0.23 | 6.47±0.09** | 1.16±0.09** | 6.88±0.09*,** |
| Endothelin | ||||
| Untreated (4) | 1.57±0.21 | 8.16±0.14 | 1.92±0.34 | 8.24±0.17 |
| Ro-318220 | 0.76±0.12** | 7.97±0.08 | 0.67±0.16** | 7.97±0.05 |
| Untreated (5) | 1.66±0.11 | 8.43±0.19 | 1.94±0.20 | 8.27±0.11 |
| Calphostin C | 0.82±0.19** | 8.08±0.15 | 0.69±0.12** | 8.07±0.18 |
| Phorbol 12,13 dibutyrate | ||||
| Untreated (8) | 2.13±0.27 | 7.28±0.09 | 1.97±0.29 | 7.32±0.11 |
| Ro-318,220 | ND | ND | ND | ND |
Mesenteric arteries were isolated from control and diabetic rats and cumulative concentration response curves to NA, ET-1 and PdBu were constructed in the absence and presence of PKC inhibitors, Ro-318220, 3 μM for 30 min, and calphostin C, 3 μM for 30 min. Number in parentheses represents number of animals. ND, not detectable.
*
P<0.05 compared to corresponding control value,
**
P<0.05 compared to corresponding untreated value (two-way ANOVA followed by Newman–Keuls post hoc test).