Figure 6.

Visualization of BODIPY®TMR-[Leu³¹, Pro³⁴]NPY in HEK293 cells expressing the rat Y₁ receptor incubated with 5 nM BODIPY®TMR-[Leu³¹, Pro³⁴]NPY for 45 min at 37°C. Nonspecific binding represents signal obtained in the presence of 1 μM [Leu³¹, Pro³⁴]NPY incubated under the same conditions with the fluorescent probe. The peptide is represented in red following HeNe laser (excitation 543 nm/emission 580 nm), and GFP-positive cells expressing low, moderate and high levels of GFP are represented in green following argon laser (excitation 488 nm/emission 510 nm). Scale bar 10 μm. All images were taken using the same setting.