Figure 9.

Visualization of 5 and 20 nM BODIPY®TMR-[Leu³¹, Pro³⁴]NPY in SK-N-MC cells and 1 and 5 nM BODIPY®TMR-[cPP(1–7), NPY(19–23), Ala³¹, Aib³², Gln³⁴]hPP in HEK293 cells expressing the rat Y₅ receptor. Cells were incubated with fluorescent probe for 45 min at 37°C. Nonspecific binding represents signal obtained in the presence of 1 μM native peptide incubated with the highest concentration of the fluorescent probe. Scale bar 10 μm. All images were taken using the same setting.