Figure 2.

Inset: Protein recovery after membrane pretreatment with ADA (1350±125 μg ml⁻¹; n=3), mock urea pretreatment (925±63 μg ml⁻¹; n=3) or urea pretreatment (520±38 μg ml⁻¹; n=3), as outlined in the Methods. ^*P<0.05; ^**P<0.01; one-way ANOVA with Bonferroni post-test. Main panel: Effects of various treatments on the binding of [³H]DPCPX (1 nM) to human A₁ receptors stably expressed in CHO cell membranes that were pretreated with 7 M urea during preparation (as indicated in Methods). [³H]DPCPX-specific binding in the absence of any treatment was 474±93 fmol mg⁻¹ protein, in the presence of 0.1 U ml⁻¹ ADA was 539±109 fmol mg⁻¹ protein, in the presence of 100 μM Gpp(NH)p was 550±114 fmol mg⁻¹ protein and in the presence of both 0.1 U ml⁻¹ ADA and 100 μM Gpp(NH)p was 494±115 fmol mg⁻¹ protein. There were no significant differences (P>0.05) between groups. All other details as for Figure 1.