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. 2005 Oct 17;146(8):1119–1129. doi: 10.1038/sj.bjp.0706419

Figure 4.

Figure 4

[3H]DPCPX saturation binding (90 min, 25°C in 50 mM Tris(HCl) buffer) to human A1 receptors stably expressed in CHO cell membranes. (a) Membranes were pretreated with 10 U ml−1 ADA during preparation (as indicated in Methods) and the assays were subsequently performed in the presence of 0.1 U ml−1 ADA. (b) Membranes were pretreated with 7 M urea during preparation (as indicated in Methods). Data points represent the mean+s.e.m. obtained from five (a) or three (b) separate experiments conducted in triplicate. The curve superimposed on the data represents the best fit of the hyperbolic term in equation (1) using the parametric values for KA and Bmax stated in Results. All other details as for Figure 1.