Table 2.
IC50 values (μM) for EGCG-induced vasorelaxation in intact and endothelium-denuded rat aortic rings precontracted with NA (1 μM), PMA (1 μM, in 1.5 mM Ca2+-containing or Ca2+-free medium) or high extracellular KCl (60 mM) in the absence (control) and presence of GB (10 μM), TEA (2 mM) or ChTX (100 nM) plus apamin (500 nM)
| Control | GB | TEA | ChTX+apamin | |
|---|---|---|---|---|
| With endothelium | ||||
| NA | 191.8±13.0 | 183.3±15.1 | 200.0±14.1 | 192.3±12.2 |
| PMA (0 mM Ca2+) | 65.6±5.1* | 58.7±4.5* | 62.7±5.1* | 66.4±3.6* |
| PMA | 178.5±12.2 | 173.7±14.5 | 185.5±14.7 | 187.6±15.0 |
| KCl | 201.2±14.9 | 189.7±13.3 | 207.7±16.7 | 191.0±12.8 |
| Without endothelium | ||||
| NA | 208.3±14.3 | 187.8±14.9 | 203.7±14.3 | 205.4±11.2 |
| PMA (0 mM Ca2+) | 67.0±5.2* | 57.3±4.0* | 63.4±5.3* | 58.1±8.3* |
| PMA | 171.6±13.8 | 177.4±14.2 | 182.4±13.1 | 186.1±14.6 |
| KCl | 193.2±15.1 | 200.1±14.7 | 202.7±15.0 | 200.3±12.9 |
Each value is the mean±s.e.m. from five experiments.
*
P<0.01 with respect to the corresponding value obtained with NA.