Figure 4.
Effect of CLT on IKur. (A) Representative voltage-dependent IKur (capacitance compensated) recorded at 0.2 Hz in a typical experiment with a 100-ms prepulse to +40 mV to inactivate Ito1, followed by 150-ms test pulses from −50 to between −40 and +50 mV after a 10-ms interval, then to −30 mV (as shown in the inset) under control conditions (a), in the presence of 10 μM CLT (b). IKur was substantially suppressed by the application of CLT, and the effect was significantly recovered by washout of the drug for 20 min (c). (B) Time-dependent effects of 10 μM CLT on IKur elicited by a 150-ms voltage step from −50 to +50 mV (as shown in the left inset) delivered every 10 s. The original IKur traces at the corresponding time points are shown in the right inset.