Figure 8.
Effects of CLT on IKs stably expressed in HEK 293 cells. (A) Time-dependent effect of 10 μM CLT on IKs-step recorded in a typical experiment with 3-s voltage step from −80 to +40 mV, then back to −40 mV as shown in the left inset. The original IKs traces at corresponding time points are shown in the right inset. (B) Voltage-dependent IKs traces recorded in a representative cell with the voltage protocol shown in the inset during control (a), in the presence of 10 μM CLT (b) and after washout (c). IKs was substantially inhibited by CLT (10 min exposure) at all voltages, and the effect recovered upon drug washout for 10 min.