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. 2007 Jan;18(1):242–252. doi: 10.1091/mbc.E06-08-0666

Figure 2.

Figure 2.

Examples of control proteins affecting neurite outgrowth when overexpressed in PC12 cells. PC12 cells were transfected with GFP-tagged ORFs and incubated for 12 h at 37°C. Thereafter cells were stimulated with NGF for 24 h, fixed and stained with ConA-Alexa647 to highlight the outline of the cells. The images show phenotypes of GFP (A) or control proteins VAMP2 (B), SNAP25 (C), Cdc42 (D), and RhoA (E) known to have an effect on neurite outgrowth upon overexpression. Each panel consists of three pictures: The left one shows ConA-Alexa647 staining; the middle one shows the overexpressed GFP-tagged protein; and the right one is a merge picture with the ConA-Alexa647 staining depicted in red and the GFP-tagged protein in green. Statistical analysis for these experiments is summarized in Supplementary Figure 1. Scale bars, 10 μm.