Figure 3.

Examples of candidate proteins affecting neurite outgrowth when overexpressed in PC12 cells. PC12 cells were transfected with GFP-tagged ORFs and incubated for 12 h at 37°C. Thereafter cells were stimulated with NGF for 24 h, fixed and stained with ConA-Alexa647 to highlight the outline of the cells. The images show phenotypes of identified effector proteins, which have similar effects on neurite outgrowth as their corresponding controls shown in Figure 2. Each panel consists of three pictures: The left one shows ConA-Alexa647 staining; the middle one shows the overexpressed GFP-tagged protein; and the right one is a merge picture with the ConA-Alexa647 staining depicted in red and the GFP-tagged protein in green. Statistical analysis for these experiments is summarized in Supplementary Figure 1. Scale bars, 10 μm.