Table 1.

AICD-dependent target genes found in this work

Gene	iFA50 clone 6	iFA50 clone 8	iFA59 clone 16	iA50 clone 6	iA50 clone 9	iA59 clone 100	iF clone 5	iF clone 15	iEGFP clone 9	iEGFP clone 22
IGFBP3
RF	5.9	4.82	4.7	5.72	5.03	4.77	1.87	1.83	1.09	0.89
SD	0.29	0.06	1.3	0.26	0.26	0.64	0.09	0.74	0.03	0.15
TAGLN
RF	3.31	4.74	3.86	5.10	1.91	2.79	1.92	1.95	0.87	0.89
SD	1.17	0.44	0.61	0.83	0.21	0.09	0.35	0.65	0.01	0.08
ACTA2
RF	2.60	3.61	2.02	2.38	1.78	1.37	1.06	0.93	0.89	1.08
SD	0.86	0.33	0.48	0.19	0.31	0.21	0.15	0.40	0.21	0.15
FN1
RF	3.19	2.25	1.7	1.36	1.07	1.63	1.25	1.09	0.80	0.72
SD	0.67	0.12	0.21	0.2	0.08	0.11	0.06	0.08	0.04	0.06
RAB3B
RF	3.13	2.06	1.59	1.47	0.78	1.49	1.43	1.48	1.01	1.16
SD	0.60	0.06	0.32	0.09	0.08	0.04	0.09	0.37	0.07	0.12
TPM1
RF	3.00	2.08	1.54	1.77	1.33	1.38	1.29	0.83	1.10	1.08
SD	0.77	0.18	0.13	0.23	0.12	0.26	0.13	0.05	0.38	0.04
SLC7A5
RF	2.35	1.66	1.54	1.27	1.69	2.08	1.23	1.33	1.04	0.96
SD	0.44	0.11	0.18	0.07	0.1	0.05	0.16	0.58	0.07	0.16
MICAL2
RF	2.24	1.53	1.45	0.76	0.67	0.93	1.08	1.08	1.18	0.97
SD	0.51	0.11	0.23	0.08	0.06	0.11	0.09	0.40	0.10	0.24

Genes were originally identified using Affymetrix GeneChips comparing induced (72 h) versus noninduced cells of two iA50 clones and one iFA50 clone. Selected genes were further analyzed using SYBR Green real-time PCR. Finally, we found eight genes that were AICD-dependent regulated. The table shows regulation factors of three iFA clones (2 iFA50 and 1 iFA59 clones), three iA clones (2 iA50 and 1 iA59 clones), two iF clones, and two iEGFP clones. Experiments were performed on two independent dates in quadruplicate. RF, regulation factor; SD, SD for calculation, method see Materials and Methods).