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Ubiquitin-independent degradation of GFP-cODC does not require Ssa1p activity, but its fusion to ΔssCPY* makes the process Ssa1p dependent. Pulse-chase analysis was done in SSA1 and ssa1-45ts cells expressing GFPuv-cODC (A), GFP-cODC (B), ΔssCG*-cODC (C), and ΔssCG*-cODC-C441A (D).
