Figure 6.
The Hsp70 Ssb class; the Hsp90 complex, Hsp104, Hsp110; small heat shock proteins Hsp26, Hsp42; and the yeast Bag1 homologue, Snl1p, are not involved in the degradation of ΔssCG*. Pulse-chase analysis was done in Δssb1Δssb2 (A), Δhsc82hsp82G170D (B), Δhsp104 (E), Δhsp26Δhsp42 (F), and Δsnl1 (G) cells expressing ΔssCG*, and cycloheximide decay experiments were performed in sti1-1 (C) and Δsse1 (D) cells expressing ΔssCG* as described in the legend to Figure 2. PGK and CPY were served as a loading control.