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Degradation of ΔssCG* requires the E2 proteins Ubc4p and Ubc5p but not the E3 ligases Doa10p and Der3p. Pulse-chase analysis was done in Δubc4Δubc5 mutant cells (A) and cycloheximide decay experiments were performed in Δdoa10Δder3 cells (B) as described in the legend to Figure 2. CPY served as a loading control.
