Figure 3.

Rab8 effectively reduces cholesterol storage in NPC1 cells with severe lipid deposition. (A) F92-116 NPC1 fibroblasts were transfected with Rab8-GFP or Rab7-GFP. (B) Filipin intensity in whole cells was quantified as in Figure 2B; Rab7-GFP (n = 73), Rab8-GFP (n = 56). (C) Filipin intensity in Lamp1-positive organelles was assayed as in Figure 2D; Rab7-GFP (n = 53), Rab8-GFP (n = 21). Values are mean ± SEM, *p < 0.05, **p ≤ 0.01. Bar, 10 μm.