Figure 6.

Rab8 is up-regulated and its membrane extractability is not compromised by severe cholesterol deposition. (A) Western blots of 15 μg of protein from cell or tissue lysates as indicated. Filters were Ponceau stained to control for equal loading. (B) Quantification of Western blots (n = 4–6 for each group, mean ± SEM, *p < 0.05, **p ≤ 0.01). (C) Cellular membranes were isolated from control (WT) and F92-116 (NPC) fibroblasts and membranes extracted with increasing amounts of Rab-GDI. The fraction of Rab protein remaining in the membranes was determined by Western blotting with anti-Rab8 antibodies. The same filters were reprobed with anti-Rab7 antibodies (mean ± SD, n = 2–3 for each GDI concentration from at least two independent experiments). **p <0.01 between Rab7 and Rab8 extractability in NPC cells.