Figure 1.

The secretory machinery in platelets from the VAMP knockout mouse strains. Platelet equivalents (5.0 × 10⁷ per lane) were loaded and analyzed by Western blotting with antibodies to the indicated proteins. (A) The v-SNARE levels in platelets from the different transgenic mouse strains (wild type: WT; VAMP-2^+/−: V2^+/−; VAMP-3^−/−: V3^−/−; VAMP-2^+/−/VAMP-3^−/−: V2^+/−/V3^−/−; VAMP-8^+/−: V8^+/−; VAMP-8^−/−: V8^−/−). (B) Western blots for the indicated t-SNARE proteins and cargo proteins in WT, V8^+/−, V8^−/− platelets.