Figure 3.

Tetanus toxin light chain does not affect secretion from permeabilized mouse platelets lacking cellubrevin/VAMP-3. Mouse platelets were prepared from VAMP-3^−/− animals, permeabilized, and treated with tetanus toxin light chain. Measurement of secretion (A) from alpha granules (■) and lysosomes (▩) was as in Figure 2. A separated set of samples was fractionated by SDS-PAGE and transferred to PVDF membrane. The membrane was stained with Ponceau S red for actin and probed by Western blotting for VAMP-2 and -8 (B).