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Ultrastructural analysis of VAMP-8−/− platelets. Platelets were prepared and adjusted to 2.5 × 108/ml. After adding 0.7 mM CaCl2, the samples were either maintained in resting state (A and B) or activated with 0.1 U/ml thrombin for 2 min (C–H). The samples were fixed and analyzed by transmission electron microscopy. (A, C, E, and G) Wild-type platelets; (B, D, F, and H) VAMP-8−/− platelets. Scale bars, 1 μm for C and D, 200 nm for others.
