Figure 1.
Wsc1 interacts with Sla1 homology domain 1 (SHD1) through the NPFDD motif. (A) Diagram of Wsc1. WSC, cysteine-rich WSC domain; TM, transmembrane domain; NPFDD, Sla1-SHD1 interaction motif. (B) SHD1 fragment (aa 471-555) from Sla1 fused to Gal4AD was tested for interaction with C-terminal cytoplasmic fragments of wild-type Wsc1 or Wsc1AAA fused to Gal4BD. Fivefold serial dilutions of exponentially growing cells were spotted onto supplemented SD with histidine (His+; no selection for interaction) or supplemented SD without histidine (His−; selection for interaction).