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. 2007 Jan;18(1):57–65. doi: 10.1091/mbc.E06-08-0721

Figure 1.

Figure 1.

Wsc1 interacts with Sla1 homology domain 1 (SHD1) through the NPFDD motif. (A) Diagram of Wsc1. WSC, cysteine-rich WSC domain; TM, transmembrane domain; NPFDD, Sla1-SHD1 interaction motif. (B) SHD1 fragment (aa 471-555) from Sla1 fused to Gal4AD was tested for interaction with C-terminal cytoplasmic fragments of wild-type Wsc1 or Wsc1AAA fused to Gal4BD. Fivefold serial dilutions of exponentially growing cells were spotted onto supplemented SD with histidine (His+; no selection for interaction) or supplemented SD without histidine (His; selection for interaction).

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