Figure 3.
Internalization of Wsc1 depends on the NPFDD signal. (A) Wsc1-GFP pep4Δ (GPY3536) or (B) Wsc1AAA-GFP pep4Δ (GPY3624) cells were metabolically labeled for 15 min, subjected to a chase for 15 min at 24°C, and then shifted to 37°C or incubated at 24°C for 60 min. Samples were collected at the designated times and divided into two aliquots that were treated with (+) or without (−) pronase. Cell lysates were prepared, Wsc1p was immunoprecipitated with GFP antibodies, and immunoprecipitates were analyzed by SDS-PAGE and autoradiography.