Figure 5.
Wsc1p constitutively recycles at 24°C. (A) Wsc1-GFP ric1Δ (GPY4112) and Wsc1AAA-GFP ric1Δ (GPY4120) cells were grown at 24°C and analyzed as in Figure 2B. (B) Wsc1-GFP vps27Δ (GPY3602) cells were grown to midlog phase, incubated with FM4-64 to label vacuoles and exaggerated prevacuolar endosomes, and analyzed by epifluorescence and DIC microscopy. Arrowheads indicate Wsc1-GFP accumulation in prevacuolar endosomes.