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. 2007 Jan;18(1):57–65. doi: 10.1091/mbc.E06-08-0721

Figure 6.

Figure 6.

Wsc1p endocytosis is required for cell wall polarity and response to cell wall stress. (A) Thin section electron micrographs of sla1Δ cells (GPY3130) containing pRS314-SLA1 (WT) or pRS314-sla1-Δshd1 (sla1-Δshd1). (B) Wsc1-GFP (GPY3527) and Wsc1AAA-GFP (GPY3544) cells. Arrows point to mother cell walls. (C) sla1Δ cells carrying pRS314-SLA1 (WT), or pRS314-sla1-Δshd1 (sla1-Δshd1) were grown in synthetic media to select for the plasmid and serial dilutions were spotted synthetic media (SD) without or with 300 ng/ml caspofungin. (D) Wild-type (WT; GPY3527) and Wsc1AAA (GPY3544) cells were grown in YPD media, and fivefold serial dilutions were spotted onto YPD or YPD containing 300 ng/ml caspofungin.

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