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. 2007 Jan;18(1):295–312. doi: 10.1091/mbc.E06-05-0461

Figure 2.

Figure 2.

Overexpression of YPT32 or YPT31 suppresses the temperature-sensitive growth defect of the cdc50-ts mutants. (A) The domain structure of Cdc50p and the amino acid substitutions in Cdc50-11p and Cdc50-162p. The black boxes indicate potential transmembrane domains. (B) Suppression of the cdc50-ts mutations by multicopy YPT32 or YPT31. cdc50-11 (YKT993) and cdc50-162 (YKT942) cells were transformed with pKT1555 (YEplac181-YPT32), pKT1554 (YEplac181-YPT31), pKT1259 (YEplac181-CDC50), or a control vector (YEplac181). Transformants were streaked onto a YPDA plate, followed by incubation at 37°C for 2 d. (C) The GTP-bound form, but not the GDP-bound form, of Ypt32p suppresses the ts growth defect of cdc50-ts mutants. cdc50-11 (YKT993) and cdc50-162 (YKT942) mutant cells were transformed with pKT1578 (YEplac181-YPT32Q72L), pKT1579 (YEplac181-YPT32S27N), pKT1555 (YEplac181-YPT32), or a control vector (YEplac181). For each plasmid, four independent transformants were streaked onto a YPDA plate, followed by incubation at 37°C for 3 d.