Table 1.
S. cerevisiae strains used in this study
| Straina | Genotype | Reference or source |
|---|---|---|
| ANS2-3A | MATαura3-52 leu2-3, 112 his3 his4 sec2-56 | Gift from A. Nakano |
| ANS6-2D | MATα ura3-52 leu2-3, 112 trp1-289 his3 his4 sec6-4 | Gift from A. Nakano |
| AKY15 | MATaade2-101 lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 Δpho8::PHO8Δ60 vps30Δ::LEU2 | Kihara et al. (2001) |
| YEF473 | MATa/α lys2-801/lys2-801 ura3-52/ura3-52 his3Δ-200/his3Δ-200 trp1Δ-63/trp1Δ-63 leu2Δ-1/leu2Δ-1 | Longtine et al. (1998) |
| YKT38 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 | Misu et al. (2003) |
| YKT259 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 CDC50-EGFP::kanMX6 | Saito et al. (2004) |
| YKT496 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 lem3Δ::TRP1 | This study |
| YKT760 | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 DNF1–13Myc::TRP1 | This study |
| YKT768 | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 DRS2-EGFP::kanMX6 | Saito et al. (2004) |
| YKT792 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 DRS2–13Myc::TRP1 | This study |
| YKT871 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 DRS2-mRFP1::TRP1 | This study |
| YKT902 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 lem3Δ::TRP1 crf1Δ::hphMX3 | This study |
| YKT903 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 KEX2-EGFP::kanMX6 | This study |
| YKT921 | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 DNF2-EGFP::kanMX6 | This study |
| YKT923 | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 lem3Δ::TRP1 DNF2-EGFP::kanMX6 | This study |
| YKT925 | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 DNF3-EGFP::kanMX6 | This study |
| YKT928 | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 crf1Δ::hphMX3 DNF3-EGFP::kanMX6 | This study |
| YKT942 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 cdc50-162::HIS3MX6 lem3Δ::TRP1 crf1Δ::hphMX3 | This study |
| YKT949 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 dnf1Δ::hphMX3 dnf2Δ::kanMX6 dnf3Δ::HIS3MX6 | This study |
| YKT951 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 rcy1Δ::kanMX4 | This study |
| YKT957 | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 VPS10-EGFP::kanMX6 | This study |
| YKT993 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 cdc50-11::HIS3MX6 lem3Δ::TRP1 crf1Δ::hphMX3 | This study |
| YKT994 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 cdc50-11::HIS3MX6 lem3Δ::TRP1 crf1Δ::hphMX3 DRS2-EGFP::kanMX6 | This study |
| YKT1000 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 cdc50-11::HIS3MX6 lem3Δ::TRP1 crf1Δ::hphMX3 KEX2-EGFP::kanMX6 | This study |
| YKT1001 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 cdc50-162::HIS3MX6 lem3Δ::TRP1 crf1Δ::hphMX3 KEX2-EGFP::kanMX6 | This study |
| YKT1004 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 cdc50-11::HIS3MX6 lem3Δ::kanMX4 crf1Δ::hphMX3 SEC7-mRFP1::TRP1 | This study |
| YKT1008b | MATα ura3 his3 trp1 leu2 cdc50-11::HIS3MX6 lem3Δ::TRP1 sec6-4 | This study |
| YKT1009b | MATaura3 his3 trp1 leu2 his4? lem3Δ::TRP1 crf1Δ::hphMX3 sec6-4 | This study |
| YKT1010b | MATα ura3 his3 trp1 leu2 his4? sec6-4 | This study |
| YKT1011b | MATaura3 his3 trp1 leu2 cdc50-11::HIS3MX6 lem3Δ::TRP1 crf1Δ::hphMX3 sec6-4 | This study |
| YKT1062 | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 DNF2-13Myc::TRP1 | This study |
| YKT1086 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 cdc50-11::HIS3MX6 lem3Δ::TRP1 crf1Δ::hphMX3 VPS10-EGFP::kanMX6 | This study |
| YKT1088 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 cdc50-162::HIS3MX6 lem3Δ::TRP1 crf1Δ::hphMX3 VPS10-EGFP::kanMX6 | This study |
| YKT1098 | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 CRF1–3HA::HIS3MX6 | This study |
| YKT1099 | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 CRF1-3HA::HIS3MX6 DNF2-13Myc::TRP1 | This study |
| YKT1100 | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 CRF1-3HA::HIS3MX6 DNF3-13Myc::TRP1 | This study |
| YKT1101 | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 CDC50-13Myc::HIS3MX6 | This study |
| YKT1102 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 rcy1Δ::hphMX4 CDC50-EGFP::kanMX6 | This study |
| YKT1310 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 lem3Δ::TRP1 crf1Δ::hphMX3 KEX2-EGFP::kanMX6 | This study |
| YNF61 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 lem3Δ::TRP1 crf1Δ::hphMX3 [GFP-SNC1 URA3 CEN] | Transformant of YKT902 with pRS416 GFP-SNC1 |
| YNF63 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1[GFP-SNC1 URA3 CEN] | Transformant of YKT38 with pRS416 GFP-SNC1 |
| YNF65 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 cdc50-11::HIS3MX6 lem3Δ::TRP1 crf1Δ::hphMX3[GFP-SNC1 URA3 CEN] | Transformant of YKT993 with pRS416 GFP-SNC1 |
| YNF67 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 cdc50-162::HIS3MX6 lem3Δ::TRP1 crf1Δ::hphMX3[GFP-SNC1 URA3 CEN] | Transformant of YKT942 with pRS416 GFP-SNC1 |
| YNF153 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 cdc50-11::HIS3MX6 lem3Δ::kanMX4 crf1Δ::hphMX3 SEC7-mRFP1::TRP1[GFP-SNC1 URA3 CEN] | Transformant of YKT1004 with pRS416 GFP-SNC1 |
| YNF784 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 dnf1Δ::hphMX3 dnf2Δ::kanMX6 dnf3Δ::HIS3MX6[GFP-SNC1 URA3 CEN] | Transformant of YKT949 with pRS416 GFP-SNC1 |
| KKT276 | MATalys2Δ0 ura3Δ0 his3Δ1 leu2Δ0 vps1Δ::kanMX4 | Gift from C. Boone |
| KKT277 | MATalys2Δ0 ura3Δ0 his3Δ1 leu2Δ0 vps4Δ::kanMX4 | Gift from C. Boone |
| YKM48 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 GAL1p-HA-CDC50::kanMX6 crf1Δ::hphMX3 | This study |
a YKT, YNF, and YKM strains are isogenic derivatives of YEF473 except for YKT1008, YKT1009, YKT1010, and YKT1011.
b YKT1008 is segregant from a cross of ANS6-2D with YKT993, YKT1009 is segregant from a cross of ANS6-2D with YKT942, YKT1010 is segregant from a cross of ANS6-2D with YKT902, and YKT1011 is segregant from a cross of YKT1008 with YKT1009.