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. 2007 Jan;18(1):189–200. doi: 10.1091/mbc.E06-05-0471

Figure 4.

Figure 4.

Increased calcium sensitivity in E-cadherin–depleted cells. (A) YFP-ZO-1 becomes mislocalized at 100 μM extracellular calcium in cells depleted of E-cadherin. MDCK T23 cells stably expressing YFP-ZO-1 were transfected with vectors for mRFP plus pSLuc or pSEcad shRNAs. After 2 d, cells were switched to medium containing either 2 or 100 μM calcium (t = 0), and YFP-ZO-1 localization was monitored for up to 60 min. (B) MDCK cells expressing control (pSLuc) or E-cadherin shRNA along with YFP, were incubated in medium containing 100 μM calcium for 15 min. Fixed cells were stained with phalloidin to visualize actin structures. (C) E-cadherin depletion causes loss of TER at 100 μM calcium. Control (pS) and E-cadherin knockdown cells (KD) were plated on Transwell filters and allowed to grow to confluence. Cells were then incubated in medium containing 2 or 100 μM calcium. TER was measured for 60 min.