Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Jan;18(1):189–200. doi: 10.1091/mbc.E06-05-0471

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2006 by The American Society for Cell Biology

PMC Copyright notice

Figure 8. — TJ biogenesis is disrupted in cells depleted of E-cadherin. E-cadherin knockdown cells fail to polarize properly after calcium switch. (A) Immunofluorescence of MDCK cells 2 d after transfection with control shRNA and YFP. (B) E-cadherin–depleted cells fixed and stained 2 d after transfection. Cells were stained for ZO-1 and β-catenin. YFP marks transfected cells. (C) Average TER after calcium switch for control (■) and knockdown cells (▴). (D) Trypsinized and replated cells were analyzed by immunofluorescence for TJ formation. Cells were fixed 7 h after replating and stained for E-cadherin and ZO-1.