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. 2007 Jan;18(1):189–200. doi: 10.1091/mbc.E06-05-0471

Figure 9.

Figure 9.

E-cadherin knockdown is partially rescued by E-cadherin–α-catenin fusion protein. (A) Live cell time-lapse imaging of YFP-ZO-1 MDCK cells after calcium switch. Cells were transfected with vectors for control or E-cadherin shRNAs or the E-cadherin–α-catenin fusion protein (Ecat). mRFP marks transfected cells. Videos are available in Supplementary Material. (B) Quantification of ZO-1 length as a measure of TJ recovery (pixels per cell). Average lengths are recorded up to 120 min after calcium switch for control cells (■) E-cadherin depleted cells (▴) and depleted cells plus an E-cadherin–α-catenin fusion protein (▾). (C) Immunoblot of cell lysates from YFP-ZO-1 cells transfected with pSLuc, pSEcadB, or pSEcadB vectors plus the E-cadherin–α-catenin fusion protein.