Figure 9.

A model for Golgi reformation. In response to BtOH, the Golgi apparatus fragments into a heterogeneous population of vesicles (A), a subset of which may redistribute into the endosomal compartment. After BtOH washout, clathrin is recruited to these vesicles, which accumulate in the juxtanuclear region of the cell, to facilitate formation of the intermediate structure (B). The tight intermediate is remodeled by a clathrin-dependent mechanism that may involve sorting of Golgi resident proteins into their respective compartments and/or removal of nonresident proteins via basal and reformation-specific anterograde transport (C). EM inset from Figure 1C.