Figure 3.

High MT depolymerization activity is not necessary for efficient sperm-induced spindle assembly. (A) The three catalytic domain mutants, GM(R522A), GM(E529A), and GM(296-308Δ), were assayed for MT depolymerization activity, quantified, and graphed as described in Figure 1, B and C. (B) The catalytic domain mutants were added back to cycled MCAK-depleted extracts (MΔ), and assayed for their ability to rescue spindle assembly as described for Figure 2. Quantification of the observed structures is graphed as the mean percentage plus the SEM from three independent experiments. An asterisk denotes a significant difference relative to add-back of GMCAK.