Figure 5.

Summary of the in vitro and extract activities of GMCAK and its various mutants. The data from each experiment are summarized with plus signs to delineate the extent of activity. Four consecutive plus signs indicate the highest activity (+ + + +) and a minus sign indicates no activity (−). ND indicates activity was not determined for that assay. Each GMCAK mutant is represented schematically based on the domain(s) deleted or mutated. The domains are as depicted in Figure 1. The catalytic domain mutants are full-length GMCAK proteins with either a point mutation (R522A or E529A) or a 13-amino acid deletion (296-308Δ) within the catalytic domain.