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Microautophagic activity of Δvtc vacuoles in vitro. Yeast cells were grown in log phase at 30°C in YPD and vacuoles were prepared at 30°C. In vitro microautophagic reactions were run with cytosol from starved wild-type cells (K91-1A) at 27°C. Data from four to nine independent experiments were averaged. Error bars, SD.
