Figure 3.

The region 83–296 of KLC1 mediates the binding to Kidins220/ARMS. (A) Scheme of the KLC1 mutants used in this study. The empty box corresponds to the heptad repeats region, whereas the gray ovals represent the TPR motifs. The putative inhibitory region for the Kidins220/ARMS–KLC interaction (aa 1-63) is marked by a dashed line. The ability of each mutant to bind Kidins220/ARMS is reported on the right: +, ≅100%; ++, ≅200%; +/−, ≅ <25%; and −, no binding. (B) The ability of in vitro ³⁵S-labeled KLC1 mutants to bind GST-KC is shown. Intensity of the bands was quantified as described in Materials and Methods. 1/25 of the starting in vitro transcription/translation mixes is loaded in T for comparison. The Coomassie staining of the SDS-PAGE gels corresponding to this experiment is available in Supplemental Figure S3B.