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. 2007 Jan;18(1):142–152. doi: 10.1091/mbc.E06-05-0453

Figure 5.

Figure 5.

KIM overexpression impairs Kidins220/ARMS trafficking in PC12 cells. (A) EGFP-Kidins220/ARMS is transported along neurites in PC12 cells. (a) Phase image of a microinjected cell. (b) Time series of the boxed area. Arrowheads indicate an EGFP-Kidins220/ARMS–positive moving carrier. Bar, 5 μm. (B–E) NGF-treated PC12 cells were coinjected with plasmids driving the expression of EGFP-Kidins220/ARMS alone or in combination with either mRFP-KIM, or mRFP-KIM(Y24A). (B) The average speed of EGFP-Kidins220/ARMS–positive carriers is decreased by mRFP-KIM expression. (C) The average number of EGFP-Kidins220/ARMS–positive carriers per neurite is not affected by the overexpression of mRFP-KIM. n = 13 neurites were analyzed for both conditions. (D) The average maximum displacement of EGFP-Kidins220/ARMS–positive carriers is reduced in mRFP-KIM–expressing cells. The analysis of the movement of EGFP-Kidins220/ARMS positive carriers is shown in E (see text and Materials and Methods for details). A 4-μm binning starting from 0 was applied. n = 24 carriers for EGFP-Kidins220/ARMS–expressing cells, n = 70 carriers for EGFP-Kidins220/ARMS– and mRFP-KIM–expressing cells; n = 74 for EGFP-Kidins220/ARMS– and mRFP-KIM(Y24A)–expressing cells. Error bars represent SEM.