Figure 7.

Overexpression of KIM reduces the phosphorylation of MAPK in PC12 cells. (A) PC12 cells were transfected with mRFP-KIM (a–c) or mRFP-KIM(Y24A) (d–f) and then fixed and immunostained for P-MAPK (c and f). mRFP-KIM–expressing cells show a reduced P-MAPK staining compared with nontransfected cells and mRFP-KIM(Y24A)–expressing cells. Transfected cells are marked with asterisks. Bars, 5 μm. (B) The mean fluorescence intensities of untransfected cells and cells overexpressing mRFP-KIM or mRFP-KIM(Y24A) were determined as described in Materials and Methods. mRFP-KIM, n = 74 cells; mRFP-KIM(Y24A), n = 63 cells; nontransfected cells, n = 204 derived from three independent experiments. Error bars represent SEM.