Table 1.
Strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| BWP17 | ura3::λimm434/ura3::λimm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG | Wilson et al. (1999) |
| CDC10-YFP | BWP17 CDC10/CDC10-YFP:URA3 | This study |
| MLC1-YFP | BWP17 MLC1/MLC1-YFP:URA3 | Crampin et al. (2005) |
| NOP1-YFP | BWP17 NOP1/NOP1-YFP:URA3 | This study |
| MET3-YFP-CDC42 | BWP17 CDC42/URA3:MET3-YFP-CDC42 | This study |
| RGA2-YFP | BWP17 RGA2/RGA2-YFP:URA3 | This study |
| BEM3-YFP | BWP17 BEM3/BEM3-YFP:URA3 | This study |
| RDI1-YFP | BWP17 RDI1/RDI1-YFP:URA3 | This study |
| rga2Δ/Δ | BWP17 rga2::HIS1/rga2::ARG4 | This study |
| bem3Δ/Δ | BWP17 bem3::HIS1/bem3::ARG4 | This study |
| rdi1Δ/Δ | BWP17 rdi1::HIS1/rdi1::ARG4 | This study |
| swe1Δ/Δ | BWP17 swe1::HIS1/swe1::ARG4 | Wightman et al. (2004) |
| rga2Δ/Δ bem3Δ/Δ | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) | This study |
| rga2Δ/Δ bem3Δ/Δrdi1Δ/Δ | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) rdi1::ura3(5′Δ)/rdi1::ura3(5′Δ) | This study |
| rga2Δ/Δ bem3Δ/Δswe1Δ/Δ | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) swe1::ura3(5′Δ)/swe1::ura3(5′Δ) | This study |
| rga2Δ/Δ CDC10-YFP | BWP17 rga2::HIS1/rga2::ARG4 CDC10/CDC10-YFP:URA3 | This study |
| rga2Δ/Δ MLC1-YFP | BWP17 rga2::HIS1/rga2::ARG4 MLC1/MLC1-YFP:URA3 | This study |
| rga2Δ/Δ NOP1-YFP | BWP17 rga2::HIS1/rga2::ARG4 NOP1/NOP1-YFP:URA3 | This study |
| bem3Δ/Δ CDC10-YFP | BWP17 bem3::HIS1/bem3::ARG4 CDC10/CDC10-YFP:URA3 | This study |
| bem3Δ/Δ MLC1-YFP | BWP17 bem3::HIS1/bem3::ARG4 MLC1/MLC1-YFP:URA3 | This study |
| bem3Δ/Δ NOP1-YFP | BWP17 bem3::HIS1/bem3::ARG4 NOP1/NOP1-YFP:URA3 | This study |
| rdi1Δ/Δ CDC10-YFP | BWP17 rdi1::HIS1/rdi1::ARG4 CDC10/CDC10-YFP:URA3 | This study |
| rga2Δ/Δ bem3Δ/ΔCDC10-YFP | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) CDC10/CDC10-YFP:URA3 | This study |
| rga2Δ/Δ bem3Δ/ΔMLC1-YFP | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) MLC1/MLC1-YFP:URA3 | This study |
| rga2Δ/Δ bem3Δ/ΔNOP1-YFP | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) NOP1/NOP1-YFP:URA3 | This study |
| rga2Δ/Δ bem3Δ/ΔrdiΔ/Δ CDC10-YFP | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) rdi1::ura3(5′Δ)/rdi1::ura3(5′Δ) CDC10/CDC10-YFP:URA3 | This study |
| rga2Δ/Δ bem3Δ/ΔrdiΔ/ΔMLC1-YFP | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) rdi1::ura3(5′Δ)/rdi1::ura3(5′Δ) MLC1/MLC1-YFP:URA3 | This study |
| rga2Δ/Δ bem3Δ/Δswe1Δ/Δ CDC10-YFP | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) swe1::ura3(5′Δ)/swe1::ura3(5′Δ) CDC10/CDC10-YFP:URA3 | This study |
| rga2Δ/Δ bem3Δ/Δ swe1Δ/ΔMLC1-YFP | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) swe1::ura3(5′Δ)/swe1::ura3(5′Δ) MLC1/MLC1-YFP:URA3 | This study |
| rga2Δ/Δ bem3Δ/ΔMET3-YFP-CDC42 | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) CDC42/URA3::MET3-YFP-CDC42 | This study |
| rdi1Δ/Δ RDI1 | BWP17 rdi1::HIS1/rdi1::ARG4/RDI1::URA3 | This study |
| rdi1Δ/Δ MET3-RDI1 | BWP17 rdi1::HIS1/rdi1::ARG4/MET3-RDI1::URA3 | This study |
| rdi1Δ/Δ pCIP10 | BWP17 rdi1::HIS1/rdi1::ARG4 rp10::URA3/RP10 | This study |
| rdi1Δ/Δ pCAEXP | BWP17 rdi1::HIS1/rdi1::ARG4 rp10::URA3/RP10 | This study |
| rga2Δ/Δ bem3Δ/Δrdi1Δ/Δ RDI1 | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) rdi1::ura3(5′Δ)/rdi1::ura3(5′Δ)/RDI1::URA3 | This study |
| rga2Δ/Δ bem3Δ/Δ rdi1Δ/ΔMET3-RDI1 | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) rdi1::ura3(5′Δ)/rdi1::ura3(5′Δ)/MET3-RDI1::URA3 | This study |
| rga2Δ/Δ bem3Δ/Δ rdi1Δ/ΔpCIP10 | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) rdi1::ura3(5′Δ)/rdi1::ura3(5′Δ) rp10::URA3/RP10 | This study |
| rga2Δ/Δ bem3Δ/Δ rdi1Δ/ΔpCAEXP | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) rdi1::ura3(5′Δ)/rdi1::ura3(5′Δ) rp10::URA3/RP10 | This study |
| rga2Δ/Δ bem3Δ/Δ MET3-RGA2 | BWP17 rga2::HIS1/rga2::ARG4/MET3-RGA2::URA3 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) | This study |
| rga2Δ/Δ bem3Δ/Δ pCAEXP | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ) rp10::URA3/RP10 | This study |
| rga2Δ/Δ bem3Δ/Δ MET3-BEM3 | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::ura3(5′Δ)/MET3-BEM3::URA3 | This study |
| CaSU64 | ura3/ura3 CDC42/cdc42::hisG PCK1-CaCDC42::hisG-URA3-hisG | Ushinsky et al. (2002) |
| CaSU64:MLC1-YFP | ura3/ura3 CDC42/cdc42::hisG PCK1-CaCDC42::hisG MLC1/MLC1-YFP:URA3 | This study |
| CaSU64:CDC10-YFP | ura3/ura3 CDC42/cdc42::hisG PCK1-CaCDC42::hisGCDC10/CDC10-YFP:URA3 | This study |
| rga2Δ/RGA2 | BWP17 rga2::HIS1/RGA2 | This study |
| bem3Δ/BEM3 | BWP17 bem3::HIS1/BEM3 | This study |
| bem3Δ/Δ rga2Δ/RGA2 | BWP17 bem3::HIS1/bem3::ARG4 rga2::ura3(5′Δ)/RGA2 | This study |
| rga2Δ/rga2Δ bem3Δ/BEM3 | BWP17rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/BEM3 | This study |
| rga2Δ/rga2 | BWP17 rga2::HIS1/rga2::ARG4 | This study |
| rga2Δ/rga2 | BWP17 rga2::HIS1/rga2::ARG4 | This study |
| bem3Δ/Δ rga2Δ/rga2 | BWP17 bem3::HIS1/bem3::ARG4 rga2::ura3(5′Δ)/ rga2::URA3 | This study |
| bem3Δ/Δ rga2Δ/rga2 | BWP17 bem3::HIS1/bem3::ARG4 rga2::ura3(5′Δ)/ rga2::URA3 | This study |
| bem3Δ/bem3 | BWP17 bem3::HIS1/bem3::ARG4 | This study |
| bem3Δ/bem3 | BWP17 bem3::HIS1/bem3::ARG4 | This study |
| rga2Δ/rga2Δ bem3Δ/bem3 | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::URA3 | This study |
| rga2Δ/rga2Δ bem3Δ/bem3 | BWP17 rga2::HIS1/rga2::ARG4 bem3::ura3(5′Δ)/bem3::URA3 | This study |
| MET3-YFP-CDC42 RDI1-6HIS | BWP17 CDC42/URA3:MET3-YFP-CDC42 RDI1/RDI1-6HIS::ARG4 | This study |