FIGURE 2.

Migration velocity. (A) Migration velocities for surfaces coated in 0.1 μg/ml E-selectin (solid bars, low-E-selectin), 1 μg/ml E-selectin (black dots, E-selectin), 1 μg/ml E-selectin + 0.5 μg/ml PECAM-1 (horizontal black stripes, low-PECAM-1), 1 μg/ml E-selectin + 1 μg/ml PECAM-1 (diagonal black stripes, moderate-PECAM-1), 1 μg/ml E-selectin + 4 μg/ml PECAM-1 (vertical black stripes, high-PECAM-1), and 1 μg/ml E-selectin + 4 μg/ml ICAM-1, (checkered, ICAM-1) at 0 (first grouping), 180 (second grouping), and 1000 s⁻¹ (third grouping). The velocities were found by dividing the contour length of the cell trajectory during the migration phase (d) by the time the cell is in its migration regime. The migration velocities are found from at least 4 d, and the error bars represent standard error. A two-way ANOVA and a least-squares means differences Tukey HSD test were performed using JMP 5.1 with α = 0.05 (^# is statistically different from ^&, and ^† is statistically different from ^‡).