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Activation of 
by 4-methylimidazole of 18O exchange catalyzed by (▴) H64W; (⋄) W5A-H64W; (•) H64G; and (□) H64G HCA II in 98% D2O. Solutions contained 25 mM of all species of CO2 at 25°C and pH or pD 7.8 (uncorrected pH meter reading). The total ionic strength of solution was maintained at a minimum of 0.2 M by addition of Na2SO4. For H64G HCA II in H2O, a fit of these data using Eq. 6 yields 
and 
. For the data in D2O, a fit of Eq. 6 yields 
and 
.
