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. 2006 Oct 27;92(2):562–570. doi: 10.1529/biophysj.106.093203

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The dependence on pH of the rate constant for ¹⁸O exchange R_H2O/[E] catalyzed by H64W HCA II and measured in the absence of buffer (▪), and in the presence of 75 mM 4-MI (○). Data representing the difference between these two sets are shown (Δ). Data were obtained at 25°C using solutions containing 25 mM of all species of CO₂. Ionic strength was maintained at a minimum of 0.2 M by addition of sodium sulfate. The solid line for the difference data was obtained by a fit to Eq. 8 resulting in for the proton donor(s) was 8.3 ± 0.1 and pK_a for the zinc-bound water was 6.7 ± 0.1.

Inline graphic — The dependence on pH of the rate constant for ¹⁸O exchange R_H2O/[E] catalyzed by H64W HCA II and measured in the absence of buffer (▪), and in the presence of 75 mM 4-MI (○). Data representing the difference between these two sets are shown (Δ). Data were obtained at 25°C using solutions containing 25 mM of all species of CO₂. Ionic strength was maintained at a minimum of 0.2 M by addition of sodium sulfate. The solid line for the difference data was obtained by a fit to Eq. 8 resulting in for the proton donor(s) was 8.3 ± 0.1 and pK_a for the zinc-bound water was 6.7 ± 0.1.