TABLE 2.

Constants of the Marcus theory for proton transfer in catalysis by variants of carbonic anhydrase

Enzyme	(kcal/mol)	wr (kcal/mol)	wp (kcal/mol)
H64W HCA II	0.3 ± 0.1	8.0 ± 0.2	8.2 ± 0.1
H64G HCA II	0.3 ± 0.1	8.5 ± 0.1	8.7 ± 0.1

Data were obtained from a fit of Eq. 9 to enhancements in R_H2O/[E] as shown in Figs. 1–3. Conditions were as in Fig. 1.