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Analytical isoelectric focusing of NTHi P5. Focusing was carried out on 0.4 mm gels of 5% acrylamide/bisacrylamide (33.7:1), 20 mM C12M, 5% pH 3–10 ampholytes formed on gel support film. The gel was stained with Coomassie brilliant Blue R250, crocein scarlet, and destained with 40% methanol, 10% acetic acid. (Lane 1) pI standards; (lane 2) NTHi P5.
