Table 1.

Chemical ischemia-induced cell injury and glutamate release in cerebrocortical slice cultures

Treatment	PI fluorescence (% of standard injury)	Glutamate (nM)
Control	11.3±1.22	217.0±19.2
Chemical ischemia (30 min)	54.7±7.45***	898.8±243.1*
Chemical ischemia (45 min)	94.5±4.67***	2373.5±280.2**
Chemical ischemia (60 min)	99.3±4.25***	4389.3±613.8**

Cultures were subjected to chemical ischemia for indicated periods, and the intensity of PI fluorescence after 24 h of postincubation (n=6 slices) and the concentration of glutamate released during ischemia (n=4 culture wells) were measured.

^*P<0.05

^**P<0.01

^***P<0.001 vs control.