Figure 4.

Activation of PKC by phorbol esters induces iNOS protein expression and NO production in J774 cells. (a) J774 cells were stimulated by LPS (10 ng ml⁻¹) and treated with PMA (100 nM) or vehicle (DMSO). After 24 h incubation, nitrite concentrations in the culture medium were measured as a marker of NO production. Values are mean±s.e.m. (n=6). (b) J774 cells were stimulated by LPS (10 ng ml⁻¹) and treated with PMA (100 nM), PDD (100 nM) or vehicle. After 24 h, incubations were terminated and immunoblots were run using antibody against iNOS. Chemiluminescent signal was quantified as described under the Methods section. Values are mean±s.e.m. (n=3). ^**P<0.01 as compared with cells treated with LPS.